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BT40/6

BT40/6 is a biological indicator strip containing Geobacillus stearothermophilus spores (1 × 10⁶ CFU) used to monitor the efficacy of steam sterilization (autoclave) cycles. It is designed for routine control, validation, and qualification of moist heat sterilizers.

INSTRUTIONS OF USE

  1. Place one BT40/6 strip inside a representative test pack or in the most challenging location of the autoclave load (hardest area for steam penetration).
  2. Run the sterilization cycle following your validated parameters.
  3. After the cycle, under aseptic conditions, transfer the strip into a sterile tube containing a suitable culture medium (e.g. Terragene’s Tryptic Soy Broth or Bionova medium).
  4. Incubate at 56–60 °C for up to 7 days, or as per your protocol.
  5. Observe for color change or turbidity indicating microbial growth:
    • No growth (no color/turbidity) → Sterilization successful.

Growth (turbidity or color change) → Sterilization failed.

AREAS OF APPLICATION

  • Monitoring and validation of steam sterilizers (autoclaves).
  • Use in:
    • Hospitals and health care facilities.
    • Pharmaceutical and biotechnological industries.
    • Laboratories and research centers.
    • Medical device and instrument manufacturers.

FEATURES & BENEFTS

  • Spore strips inoculated with Geobacillus stearothermophilus (1×10⁶ CFU).
  • Excellent resistance and reproducibility according to ISO 11138-3.
  • Ideal for routine control, qualification, and validation of autoclaves.
  • Long shelf life with stable spore population.
  • Compatible with various culture media.
  • Reliable indication of sterilization efficiency.

COMPOSITION

  • Paper carrier strip inoculated with Geobacillus stearothermophilus spores.
  • Packaged in a protective glassine envelope to maintain sterility and stability.

PRECAUTIONS OF USE

  • Store at 15–30 °C and dry conditions.
  • Do not expose to sterilizing agents or moisture before use.
  • Handle aseptically when transferring to growth media.
  • Do not use damaged or expired strips.
  • Dispose of after incubation according to biohazard waste procedures.

PACKING TYPES

  • Packing: Box of 100 strips (in glassine envelopes).

PRODUCT CODE

  • Product Code: BT40/6